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Effects of Decoction on plasma proteome in cirrhosis: preliminary experimental study with rats

LIU Jie, WANG Jiyao, WEI Liming, LU Ye, Jin Hong

《医学前沿（英文）》 2008年 第2卷 第1期   页码 39-44 doi: 10.1007/s11684-008-0008-z

摘要： The aim of this paper is to study the effects of Decoction on the plasma proteome in cirrhotic rats. Twenty-six male Sprague-Dawley (SD) rats were randomly divided into three groups: cirrhotic model group ( = 10), treated with CCl (CCl/olive oil: v/v = 1:1); Decoction intervention group ( = 10), treated with CCl+ Decoction; and normal control group ( = 6), treated with olive oil only. After 8 weeks, blood samples were collected from the inferior vena cava to undergo bi-dimensional electrophoresis (2DE) and analysis by PDQuest 7.3 software. Differential protein spots were cut, enzyme hydrolysis was conducted, and peptide fragments extracted from the mixture underwent mass spectrometry (MS) with MALDI-TOF-TOF-MS. The liver fibrogenesis was assessed using a digital image analysis instrument of Masson’s trichrome stained sections. The fibrosis area of the Decoction was (8.9 ± 3.7)%, significantly smaller than that of the cirrhotic model group [(12.4 ± 4.7)%, < 0.05]. Ten markedly changed protein spots were identified by MALDI-TOF-TOF-MS. Eight of the 10 proteins, including plasma glutathione peroxidase, plasma glutathione peroxidase precursor, prealbumin, haptoglobin, apolipoprotein A-IV precursor, complement C4, inter-alpha-inhibitor H4 heavy chain, and serine/threonine-protein kinase microtubule-affinity regulating kinase 1 (MARK1) were expressed very lowly in the cirrhotic model group while they were expressed highly in the Decoction group. The expression of liver regeneration-related protein LRRG03 and vimentin increased in the cirrhotic model group, and reduced in the Decoction group. Some proteins related to oxidative stress, cell proliferation and transformation have changed in the plasma of cirrhosis induced by CCl. Decoction promotes protein synthesis and plays an anti-fibrotic role by anti-oxidation and accommodation of cell proliferation and transformation.

关键词： Decoction intervention     serine/threonine-protein     underwent     Decoction     glutathione peroxidase    

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Mechanism of hepatocellular damage in rat caused by low serum selenium

LU Yi, QU Bo, LIU Chang, YU Liang, Liu Xuemin, WANG Haohua, JIANG An, ZHANG Xiaogang

《医学前沿（英文）》 2008年 第2卷 第3期   页码 255-258 doi: 10.1007/s11684-008-0048-4

摘要： The aim of this paper is to investigate the mechanism of hepatocellular damage in rats caused by low serum selenium. Thirty six rats were randomly divided into 2 groups: group A (fed with low-selenium diet from the Keshan Disease area with the content of selenium being 0.017 mg/kg); group B [fed with selenium-supplemented diet and 0.3 mg/L selenium (NaSeO) was added to the drinking water]. Both were respectively fed for 12 weeks. At the end of the 12 week, the levels of serum selenium, glutathione peroxidase (GPX) and malondialdehyde (MDA) in hepatic tissue were measured; the hepatocellular ultrastructure and apoptosis were observed as well. The levels of serum selenium and GPX in group A were markedly lower than those in group B. MDA level in group A was significantly higher than that in group B. Under the electron microscope (EM), the mitochondria were remarkably changed in group A. The rate of liver cell apoptosis appeared much higher in group A as well. It indicated that the damage caused by selenium deficiency was through the process of oxidation. Selenium deficiency led to apoptosis of hepatocytes where oxidative damage to mitochondria might be the cause.

关键词： process     glutathione peroxidase     hepatocellular damage     group     Keshan Disease    

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Response of peroxidase and catalase to acid rain stress during seed germination of rice, wheat, and rape

WANG Lihong, HUANG Xiaohua, ZHOU Qing

《环境科学与工程前沿（英文）》 2008年 第2卷 第3期   页码 364-369 doi: 10.1007/s11783-008-0053-5

摘要： Seed germination of plants with various acid-resistance display different responses to acid rain. To understand the reason why such differences occur, the effects of simulated acid rain (pH 2.5–5.0) on the activities of peroxidase (POD) and catalase (CAT) during seed germination of rice (), wheat (), and rape ( var. ) were investigated. Results indicated that the maximum change in activities of CAT and POD by acid rain treatment with different acidity and time in relation to the referent treatment without acid rain, was in the order: rice (28.8%, 31.7%) < wheat (34.7%, 48.3%) < rape (79.3%, 50.0%). The pH level for which the treatment with acid rain did not cause significant difference ( < 0.05) was in the order: rice (3.5) > wheat (4.0) > rape (5.0). Moreover, the change in activity of POD was higher than that of CAT, which showed that POD was more sensitive to acid rain stress than CAT. The difference in the ability of POD and CAT in removing free radicals was one reason why the germination indexes of these three species behaved differently.
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Roles of glutathione and L-cysteine in the biomimetic green synthesis of CdSe quantum dots

Ling-Li Li, Yin-Hua Cui, Jie-Jie Chen, Han-Qing Yu

《环境科学与工程前沿（英文）》 2017年 第11卷 第6期 doi: 10.1007/s11783-017-0948-0

摘要： Biological synthesis of quantum dots (QDs) as an environmental-friendly and facile preparation method has attracted increasing interests. However, it is difficult to distinguish the roles of bio-thiols in QDs synthesis process because of the complex nature in organisms. In this work, the CdSe QDs synthesis conditions in organisms were reconstructed by using a simplified in vitro approach to uncover the roles of two small bio-thiols in the QDs formation. CdSe QDs were synthesized with glutathione (GSH) and L-cysteine (Cys) respectively. Compared with Cys at the same molar concentration, the CdSe QDs synthesized by GSH had a larger and broader particle size distribution with improved optical properties and crystal structure. Furthermore, quantum chemical calculations indicate that the stronger Cd2+ binding capacity of GSH contributed a lot to the CdSe QDs formation despite of the greater capability Cys for selenite reduction. This work clearly demonstrates the different roles of small thiols in the Cd2+ stabilization in the environment and biomimetic QDs synthesis process.

关键词： CdSe     Quantum dots (QDs)     Biomimetic synthesis     Bio-thiols     Glutathione (GSH)     Cysteine (Cys)    

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Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalyticpocket and the application of peroxidase-coated yeast cell walls

Karla Ilić Đurđić, Raluca Ostafe, Olivera Prodanović, Aleksandra Đurđević Đelmaš, Nikolina Popović, Rainer Fischer, Stefan Schillberg, Radivoje Prodanović

《环境科学与工程前沿（英文）》 2021年 第15卷 第2期 doi: 10.1007/s11783-020-1311-4

摘要： Abstract • Mutations in Lignin peroxidase Trp171 environment improved azo dyes degradation. • Expression on yeast cell surface and cell lysis allowed reusability of biocatalyst. • Aga2-LiP chimeric variants were characterized. The enzymatic degradation of azo dyes is a promising alternative to ineffective chemical and physical remediation methods. Lignin peroxidase (LiP) from Phanerochaete chrysosporium is a heme-containing lignin-degrading oxidoreductase that catalyzes the peroxide-dependent oxidation of diverse molecules, including industrial dyes. This enzyme is therefore ideal as a starting point for protein engineering. Accordingly, we subjected two positions (165 and 264) in the environment of the catalytic Trp171 residue to saturation mutagenesis, and the resulting library of 104 independent clones was expressed on the surface of yeast cells. This yeast display library was used for the selection of variants with the ability to break down structurally-distinct azo dyes more efficiently. We identified mutants with up to 10-fold greater affinity than wild-type LiP for three diverse azo dyes (Evans blue, amido black 10B and Guinea green) and up to 13-fold higher catalytic activity. Additionally, cell wall fragments displaying mutant LiP enzymes were prepared by toluene-induced cell lysis, achieving significant increases in both enzyme activity and stability compared to a whole-cell biocatalyst. LiP-coated cell wall fragments retained their initial dye degradation activity after 10 reaction cycles each lasting 8 h. The best-performing mutants removed up to 2.5-fold more of each dye than the wild-type LiP in multiple reaction cycles.

关键词： Bioremediation     Enzyme immobilization     Protein engineering     Yeast surface display.    

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Analysis of two new degradation products of arsenic triglutathione in aqueous solution

Feng ZHAO, Yuchen CHEN, Bin QIAO, Jing WANG, Ping NA

《化学科学与工程前沿（英文）》 2012年 第6卷 第3期   页码 292-300 doi: 10.1007/s11705-012-1208-2

摘要： Inorganic arsenicals, including arsenite (As ) and arsenate (As ), are well-known human carcinogens. Recently, studies have indicated that arsenic triglutathione (As(GS) ) is unstable in an aqueous solution. The present study was designed to evaluate the degradation mechanism of As(GS) in an aqueous solution using high-performance liquid chromatography-electrospray ionisation mass spectrometry (HPLC-ESI-MS). Based on the fragments obtained from MS and MS , we identified two new compounds: one was an isomer of glutathione (GSH), and the other was a product from the cleavage of the glutamyl of oxidised glutathione (GSSG). The isomerization of GSH resulted in the loss of its function such as detoxification of many reactive metabolites. The formation of the two new compounds affected the ratio of GSH/GSSG, and thus may affect the antioxidant and detoxification of GSH/GSSG in mammalian cells.

关键词： arsenic triglutathione     glutathione     HPLC-ESI-MS    

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标题 作者 时间 类型 操作

Effects of Decoction on plasma proteome in cirrhosis: preliminary experimental study with rats

LIU Jie, WANG Jiyao, WEI Liming, LU Ye, Jin Hong

期刊论文

Mechanism of hepatocellular damage in rat caused by low serum selenium

LU Yi, QU Bo, LIU Chang, YU Liang, Liu Xuemin, WANG Haohua, JIANG An, ZHANG Xiaogang

期刊论文

Response of peroxidase and catalase to acid rain stress during seed germination of rice, wheat, and rape

WANG Lihong, HUANG Xiaohua, ZHOU Qing

期刊论文

Roles of glutathione and L-cysteine in the biomimetic green synthesis of CdSe quantum dots

Ling-Li Li, Yin-Hua Cui, Jie-Jie Chen, Han-Qing Yu

期刊论文

Improved degradation of azo dyes by lignin peroxidase following mutagenesis at two sites near the catalyticpocket and the application of peroxidase-coated yeast cell walls

Karla Ilić Đurđić, Raluca Ostafe, Olivera Prodanović, Aleksandra Đurđević Đelmaš, Nikolina Popović, Rainer Fischer, Stefan Schillberg, Radivoje Prodanović

期刊论文

Analysis of two new degradation products of arsenic triglutathione in aqueous solution

Feng ZHAO, Yuchen CHEN, Bin QIAO, Jing WANG, Ping NA

期刊论文